Aseptic Technique in Rodent Surgery : Batch Surgery

Key Points

  • Aseptic techniques can be maintained throughout a series of procedures but the use of an assistant becomes almost essential.
  • After completing the first procedure, the surgeon lifts and disposes of the sterile drape.
  • The assistant then moves the animal to a recovery area, or directly into a warm incubator.
  • The next animal to undergo surgery is then anaesthetised, clipped, positioned on the table and a skin preparation undertaken by the assistant.
  • Ideally the surgeon will use a new set of instruments, but it is acceptable to use a hot bead steriliser to re-sterilise the tips of the instruments.
  • If a new set of instruments is to be used, the surgeon should re-glove before handling them.
  • This can be done without the need to re-gown.
  • The surgeon re-drapes the animal using a new sterile drape and carries out surgery.

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A comprehensive guide to help research workers apply best practice in aseptic surgical techniques in laboratory rodents.

The material was developed by Newcastle University with the support of the NC3Rs.

National Centre for the Replacement, Refinement and Reduction of Animals in Research. Newcastle University

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