Aseptic Technique in Rodent Surgery : The Surgical Procedure

Key Points

  • Check that all the instruments needed are present and arranged in a convenient order before undertaking surgery.
  • After use, each instrument should be placed back on a sterile drape.
  • Maintaining aseptic technique is much easier, and procedures will be completed more rapidly when using an assistant.
  • If an assistant is not available throughout the procedure, non-sterile surfaces that may need to be handled (for example an anaesthetic vaporiser) can be covered with a sterile wrapping, or touched using a large sterile swab which is then discarded.

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A comprehensive guide to help research workers apply best practice in aseptic surgical techniques in laboratory rodents.

The material was developed by Newcastle University with the support of the NC3Rs.

National Centre for the Replacement, Refinement and Reduction of Animals in Research. Newcastle University

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