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Oral Gavage in the Mouse
Material can be administered orally using a number of different techniques, although gavage using a stomach tube is the most widely used. To minimise the risk of adverse effects associated with this procedure, it is important that the operator is skilled both in the technique and the restraint method needed…
Subcutaneous Injection in the Rat
Subcutaneous administration of material often causes minimal pain or discomfort, provided the material is non-irritant, has a near-neutral pH, and is not excessively hypertonic or hypotonic…
View Full TutorialIntravenous Injection in the Rat
Intravenous administration of material can be technically difficult, and use of a restraining device is often required. These should be selected carefully to be an appropriate size for the animal to be injected. Too small a device can result in injuries to the animal, and can interfere with respiratory movements. Too large a restrainer can also result in injury, caused by movements during restraint…
View Full TutorialIntramuscular Injection in the Rat
Intramuscular injections are made into the front or back of the thigh in all small rodents. In the rat and guinea pig the muscle mass is usually sufficient for accurate administration of small volumes of material (e.g. <0.1ml/kg)…
Oral Gavage in the Rat
Material can be administered orally using a number of different techniques, although gavage using a stomach tube is the most widely used. To minimise the risk of adverse effects associated with this procedure, it is important that the operator is skilled both in the technique and the restraint method needed…
Intraperitoneal Injection in the Rat
Although widely used as a means of administering substances, particularly injectable anaesthetics, this is an inherently unreliable technique, since inadvertent injection of some material into the gut, abdominal fat and subcutaneous tissues is a relatively frequent occurrence…
View Full TutorialSubcutaneous Injection in the Mouse
Subcutaneous administration of material often causes minimal pain or discomfort, provided the material is non-irritant, has a near-neutral pH, and is not excessively hypertonic or hypotonic…
View Full TutorialIntravenous Injection in the Mouse
Intravenous administration of material can be technically difficult, and use of a restraining device is often required. These should be selected carefully to be an appropriate size for the animal to be injected. Too small a device can result in injuries to the animal, and can interfere with respiratory movements…
View Full TutorialIntraperitoneal Injection in the Mouse
Although widely used as a means of administering substances, particularly injectable anaesthetics, this is an inherently unreliable technique, since inadvertent injection of some material into the gut, abdominal fat and subcutaneous tissues is a relatively frequent occurrence…
View Full TutorialHandling and Restraint of the Animal
Almost all laboratory animals can be restrained safely and humanely provided they are handled correctly. All animals benefit from being first accustomed to being handled, and this can be done during the period of acclimatisation needed before they are used on a research procedure.
View Full TutorialADMINISTRATIONOF SUBSTANCES
A series of tutorials to assist research workers develop their skills in the administration of substances to laboratory animals.
The material was developed by Newcastle University with the support of the IAT and the NC3Rs.
